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In silico identification of microRNAs and their target genes in watermelon (Citrullus lanatus)

Show simple item record Zakeela, M. C. A. Safeena, M. I. S. Komathy, T. 2019-07-18T07:40:47Z 2019-07-18T07:40:47Z 2019-03
dc.identifier.citation Scientia Horticulturae, (252)27: 55-60. en_US
dc.description.abstract Watermelon (Citrullus lanatus) is an annual herbaceous crop, mainly grown for its fleshy fruit, which is an excellent source of vitamins and therefore fetches a huge market demand all over the world. Yield of watermelon can be improved by increasing the productivity that relies on plant growth, development and stress response. MicroRNAs (miRNAs), a group of short (∼22 nt), noncoding, endogenous and evolutionary conserved post-transcriptional regulatory RNAs, play a major role in various biological and metabolic processes associated with growth, development and stress response. In this study, we deployed a comparative genomics-based approach using expressed sequence tags (ESTs) to identify and characterize potential miRNAs and their target genes in watermelon. Three potential miRNAs (cla-miR-2515, cla-miR482d and cla-miR-8461) were predicted from 17,594 publicly available watermelon EST sequences, according to a series of filtering criteria used in the protocol. Moreover, 21 potential mRNA targets encoding for 19 different proteins linked to various metabolic processes such as inositol-3-phosphate synthase activity, metal ion binding and oxidoreductase activity were identified in watermelon genome, based on perfect or nearly perfect complementarity of miRNAs with their targeted mRNA sequences. Phylogenetic analysis of these miRNAs revealed that cla-miR8461 and cla-miR2515 are unique miRNAs closely related to Schistosoma mansoni and Drosophila pseudoobscura, respectively. en_US
dc.language.iso en_US en_US
dc.publisher Elsevier en_US
dc.subject In silico identification en_US
dc.subject Citrullus lanatus en_US
dc.subject miRNA en_US
dc.subject Stress response en_US
dc.subject Expressed sequence tag (EST) en_US
dc.title In silico identification of microRNAs and their target genes in watermelon (Citrullus lanatus) en_US
dc.type Article en_US

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