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A Study on binding of hydroxamic acid derivative to DNA: experimental and computational approach

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dc.contributor.author Jumani, ALF
dc.contributor.author Safeena, MIS
dc.contributor.author Senthilnithy, R
dc.date.accessioned 2017-01-13T06:22:30Z
dc.date.available 2017-01-13T06:22:30Z
dc.date.issued 2016-12-29
dc.identifier.citation Proceedings of Fifth Annual Science Research Sessions 2016 on "Enriching the Novel Scientific Research for the Development of the Nation" pp.217-224 en_US
dc.identifier.isbn 9789556271027
dc.identifier.uri http://ir.lib.seu.ac.lk/handle/123456789/2098
dc.description.abstract Small Gilligan molecules bind to DNA and artificially alter and/or inhibit the functioning of DNA. These small ligand molecules act as drug when alteration or inhibition of DNA function is required to cure or control a disease.The binding interaction of N-phenyllauroylhydroxamic acid with CTDNA was measured by four methods, (i) UV spectroscopic method, (ii) fluorescence spectroscopic method, (iii) viscosity Measurement and (iv) molecular docking. It showed that Nphenyllauroylhydroxamic acid-DNA complex has high absorption intensity than compound only and significant quenching of fluorescence intensity for the N-phenyllauroylhydroxamic-DNA complex. The values of binding constant, K, is 3.43x 10-2nd Stern Volmer constant Ksv is 4.8 x102 ng-1μl obtained by UV absorption and fluorescence spectral methods, respectively. The binding interaction is further confirmed by the increase in relative viscosity of CT-DNA. The molecular docking of N-phenyllauroylhydroxamic acid with the DNA confirmed to be a strong binder with DNA in the mode of groove. en_US
dc.language.iso en_US en_US
dc.publisher Faculty of Applied Sciences, South Eastern University of Sri lanka en_US
dc.subject Hydrodynamic en_US
dc.subject Calf thymus DNA (ct-DNA) en_US
dc.subject Molecular docking en_US
dc.title A Study on binding of hydroxamic acid derivative to DNA: experimental and computational approach en_US
dc.type Article en_US


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