dc.contributor.author |
Safeena, M. I. S. |
|
dc.date.accessioned |
2019-11-26T11:20:44Z |
|
dc.date.available |
2019-11-26T11:20:44Z |
|
dc.date.issued |
2019-11-27 |
|
dc.identifier.citation |
9th International Symposium 2019 on “Promoting Multidisciplinary Academic Research and Innovation”. 27th - 28th November 2019. South Eastern University of Sri Lanka, University Park, Oluvil, Sri Lanka. pp. 373-381. |
en_US |
dc.identifier.isbn |
978-955-627-189-8 |
|
dc.identifier.uri |
http://ir.lib.seu.ac.lk/handle/123456789/3942 |
|
dc.description.abstract |
Ibuprofen belongs to a class of drugs called non-steroidal anti-inflammatory drugs (NSAIDs) with
various pharmacological effects. A set of spectroscopic (in-vitro) and molecular docking (in-silico)
studies were accounted to illustrate the binding mode of ibuprofen with calf thymus DNA (ct-DNA),
in order to predict its possibility to be a ‘DNA binder’ which is one of the features expected in
anticancer and antitumor drug designing. Both the UV-Visible and Fluorescence spectroscopic results
denote that, there is a drug-DNA complex formation with binding constants of 1.58 x 105 M-1 and 2
x 10-3 μl ng-1 respectively. These values suggest that ibuprofen having intercalative mode of
interaction with ct-DNA. As a confirmation of these results, it was further validated through relative
specific viscosity measurements of ct-DNA dug complex. It was revealed an increment while mixing
DNA with the drug in ascending order. Molecular docking studies further complemented the
experimental results. |
en_US |
dc.language.iso |
en_US |
en_US |
dc.publisher |
South Eastern University of Sri Lanka, University Park, Oluvil, Sri Lanka |
en_US |
dc.subject |
NSAIDs |
en_US |
dc.subject |
DNA binding |
en_US |
dc.subject |
Fluorescence spectroscopic |
en_US |
dc.subject |
Molecular docking |
en_US |
dc.title |
Ibuprofen binds DNA: evidenced through fluorescence spectroscopic and molecular docking |
en_US |
dc.type |
Article |
en_US |