Abstract:
-Efforts to optimize protocols, explore
alternative substances, and advance research are critical
to making tissue culture a sustainable and cost-effective
method for mass plant propagation. Hence, the present
study was conducted at the Floriculture Research and
Development Unit of the Royal Botanical Gardens,
Peradeniya. The objective was to evaluate the potential of
using Aloe vera (Aloe barbadensis Miller) and Kesibissan
(Cyclea peltata L.) as nutrient additives and gelling
medium for Anthurium tissue culture. The experiment
was designed using a Complete Randomized Design with
seven treatments, and each experiment was replicated 15
times.The number of leaves, shoots and roots per plantlet and the
explant height were measured. Based on the results, our study
found that supplementing Aloe vera and Kesibissan
extracts had a substantial impact on the growth
parameters of Anthurium explants after one month of
incubation period. Kessibissan extracts performed well on
Anthurium explant growth, especially in terms of the
number of leaves, roots, and plantlet height. Meanwhile,
the treatment involving 700 ml of MS media and 300 ml
of Aloe vera extract proved to be the most successful for
Anthurium, resulting in improved leaf, root, and shoot
development. Overall, our findings give useful
information for improving the growth of this attractive
plant and may have applications for horticultural
operations, highlighting the potential benefits of natural
extracts in plant tissue culture.
