Abstract:
Neuroinflammation, characterized by inflammatory responses within the brain and
spinal cord, plays a pivotal role in various neurological diseases, including
neurodegenerative conditions, psychiatric disorders, pain syndromes, stroke, and
traumatic brain injuries. This inflammatory process is primarily driven by cells such as
astrocytes, microglia, immune cells, and the blood-brain barrier. The interplay between
various receptors and signalling pathways is of great interest in understanding the
underlying mechanisms of neuroinflammation. In this study, we aimed to investigate
the potential interaction between Sphingosine-1-Phosphate Receptor-3 (S1PR3), a G
protein-coupled receptor, and Toll-Like Receptor-4 (TLR4), a pattern recognition
receptor, within C6 glioma cells (Rattus norvegicus) under conditions of
neuroinflammation and the presence of S1PR3 receptors. Our experimental approach
involved several key steps.C6 glioma cells were cultured, and changes in cell growth
and morphology were meticulously observed. Subsequently, these cells were utilized
for RNA extraction assays. To verify the expression of S1PR3 and TLR4 in the cultured
C6 glioma cells, fluorescence imaging was performed, revealing the presence of S1PR3
in modified cells under the U-FBNA filter. In future work, these validated cells can
serve as a valuable platform for exploring the actions of Sphingosine-1-Phosphate
receptors in the context of neuroinflammation. The expression levels of the receptors
and relevant cytokines will be analysed using Reverse Transcriptase-Polymerase Chain
Reaction.This study contributes to our understanding of the molecular and cellular
mechanisms underlying neuroinflammation, with a particular focus on the potential
interplay between S1PR3 and TLR4 in C6 glioma cells, offering insights into their roles
in the pathophysiology of neurological diseases.
