Abstract:
antibiotics, has led to an increasing demand for the exploration of novel antibacterial
compounds, particularly those sourced from botanical origins. This study aims to
explore the antibacterial potential of phytochemicals obtained from Artocarpus
heterophyllus and Mikania micrantha, two indigenous Sri Lankan botanical sources.
Synergistic and antagonistic effects were examined by combining A. heterophyllus with
a botanical source and a chemical source. This study was conducted to assess the
combined effects of these phytochemicals with sodium salt of metatitanic acid (MTA)
against certain bacterial strains. The sodium salt of metatitanic acid, a chemical that
possesses superior antibacterial properties in previous studies, was used as the chemical
source in this study. Additionally, M. micrantha, a plant commonly known as
‘wathupalu’ and utilized in traditional medicinal practices, was used as the botanical
source. The A. heterophyllus plant parts (leaves, seeds, stripes and peels) were cleaned,
dried and pulverised, and then the aqueous extract was obtained. Similarly, M.
micrantha leaves were cleaned, dried and crushed in order to obtain an aqueous extract.
The crude extracts were individually assessed for antibacterial activity against
Staphylococcus aureus (ATCC 25923) and Pseudomonas aeruginosa (ATCC 27853)
using antibiotic sensitivity test (ABST), by well diffusion method employed using
Muller-Hinton agar as the growth medium. The plates were incubated for 24 hours at
37°C. The positive control used was Gentamicin and the negative control used was
sterile water. The crude extract of A. heterophyllus was combined with M. micrantha
extract in 1:1 ratio and was tested against the same species for enhanced antibacterial
properties. Similarly, the crude extract of A. heterophyllus was mixed with the sodium
salt of MTA and ABST assay was conducted to analyze and compare the antibacterial
properties. ANOVA and Post hoc analysis performed using SPSS 27th version revealed
that MTA exhibits stronger antibacterial property (23.5±0.4 mm), while the
combination of MTA and A. heterophyllus also showed a synergistic effect (24±1.7
mm) against S. aureus. Moreover, the analysis against P. aeruginosa shows that M.
micrantha exhibits notable antibacterial properties against this species (27.4±3.6
mm). The statistical significance for all comparisons was established at the p<0.05
level. In conclusion, the sodium salt of MTA, M. micrantha, and their combination with
A. heterophyllus exhibit potential antibacterial properties.
