Abstract:
Amides can be particularly used as an intermediate for the drugs as well as
pharmaceuticals. In this research work eleven benzanilide derivatives were synthesized
using benzoyl chlorides and aniline derivatives and characterized. In vitro antibacterial,
antifungal and antioxidant activity of the synthesized compounds were evaluated. In
order to check the purity of the synthesized compounds Thin layer chromatography was
used. Structural characterization was done by using FTIR (Fourier-transform infrared
spectroscopy) and GC-MS (mass spectrometry).The disc diffusion method was used to
evaluate the antimicrobial and antifungal activities and corresponding activities were
calculated by measuring the diameter of the zone of inhibition (ZOI) of microbial
growth around the disc paper. The antimicrobial assay was determined against Bacillus
spp and Escherichia coli while the antifungal assay targeted Aspergillus spp and
Trichoderma spp. In both experiments Gentamycin served as a positive control and
DMSO (Dimethyl sulfoxide) was utilized as the negative control. The antioxidant
activity of the synthesized compounds was tested using DPPH assay compared to the
standard antioxidant ascorbic acid. Based on the observed results the compounds
1,2,3,4,6,7,8 and 10 were found to inhibit the growth of the Bacillus spp and
Escherichia coli with ZOI ranging from 6.3-10.3 mm and 6.8-10.1 mm respectively.
Compounds 5,9 and 11 were found to be inactive against tested bacteria. The compound
6 N-(3,5bis(trifluoromethyl)phenyl) benzamide found to inhibit the growth of
Aspergillus spp with the ZOI 7.1 mm. Compounds 1,2,3,4,5,7,8,9,10 and 11 were
showed no inhibition against Aspergillus spp. None of the synthesized compound show
antifungal activity against Trichoderma spp or antioxidant activity by using DPPH
assay.
