Abstract:
Micropropagation is widely used for potatoes (Solanum tuberosum L.) as a
viable option for propagation. This research investigates alternative media
for in vitro methods to propagate potatoes. Three treatments were
investigated. The MS medium, serving as the control, was composed of 1
mg/l BAP (6-Benzylaminopurine), 0.1 mg/l NAA (Naphthalene acetic acid),
30 g/l sugar, 0.1 g/l Myo-inositol, and 1 g/l charcoal, while the other two
treatments, Albert’s solution and a novel media (Kodi mix, KM), were
supplemented at the rates of 2 g/l to the above media. All three treatments
were replicated five times, employing a completely randomized block design
(CRD). Surface-sterilized potato shoots with one node were introduced into
different types of media for regeneration. Growth and developmental data
were collected at 3, 4, and 5 weeks of in vitro culture. Subsequently, sterilized
sand and coir dust in a 1:1 ratio were utilized for plant acclimatization, and
the health of the plants was assessed. Our results indicated that Albert’s
solution (28/plant) improved root development (number + length), while KM
displayed the lowest numbers (13/plant). However, shoot length, number of
roots, leaves, and internode showed significant differences among treatments
in the MS treatment, while KM promoted marginally. Moreover, treatment
with Albert’s solution produced the highest number of healthy plants at
83.6%, followed by MS at 79.6%, and KM at 51.1%, respectively, at the postacclimatization stage. Hence, we conclude that both Albert’s and MS media
promote the overall performance of potato tissue-cultured plantlets, while the
novel Kodi mix medium promotes the shoot growth and development
marginally and minimal root development in potato tissue-cultured plantlets.
