Abstract:
The majority of the drug industry's work involves the discovery and analysis of
bioactive substances. Drugs derived from plants have more potential than those derived from
chemicals. Some pesticide compounds are prohibited from importation into Sri Lanka.
Therefore, it is more convenient to search for natural insecticides than synthetic chemicals. The
pest known as Sitophilus oryzae ruins rice kept in storage.There are plenty of medicinal plants
which act as therapeutic agents. Using conventional herbal remedies including Lantana camera
(leaf), Carica papaya (seeds), Ricinus communis (leaves), Calotropis giganta (flowers), and
Gliricidia sepium (leaves), and methanol as solvents (1:10 w: v), it has been achieved to
identify plants' insecticidal properties. The best pesticide plant, among the selected plants,
according to a one-week observation on mortality, was Gliricidia sepium (leaves). To display
the results of the total experiment, which also included information on the control test, a
survival analysis test was used. With the aid of a gradient solvent system, chromatographic
separations were used to fractionate Gliricidia sepium leaf extracts. Overall, twelve fractions
were gathered, and a mortality test took place to figure out the most bioactive fractions under
lab environments. The potential anti-Sitophilus oryzae ability was demonstrated from fraction
eleven. Continuously, instrumental analysis was used to describe and pinpoint a specific
bioactive component. In accordance with the results of the FTIR analysis, four significant
peaks were found, and they corresponded to the presence of the O-H bond, C-H bond
stretching, -CH3 bending, and C-O stretching at frequencies of 3338.14cm-1, 2939.41 cm-1,
1411.59 cm-1, and 1011.08 cm-1 respectively. Additional GC-MS analysis, when combined
with NIST library data, revealed the potential structure and mass fragments of anti Sitophilus
oryzae. Briefly, the mass to charge proportions for [C7H3]11˙, [C7H11O3]3˙, and [C7H8O]6˙ have
been observed in the CG-MS spectrum at m/z 87.0000, 143.071, and 108.508 respectively.
Finally, in my research, I found that 4-c-methyl-myo-inositol was the anti-Sitophilus oryzae
drug responsible.
