Abstract:
N-substituted phthalimides are biologically active chemicals with a broad range of
applications. In this present research work, eight 3-Nitroisoindoline-1,3-dione
analogues were synthesized and characterized. Furthermore, their antibacterial,
antifungal and antioxidant activities were evaluated in vitro. Synthesized
compounds were purified using the recrystallization process and the purity was
confirmed by thin-layer chromatography. The structures were characterized by
FTIR and mass spectroscopic analysis. Using the Disc diffusion method, synthetic
3-Nitroisoindoline-1,3-dione analogues were tested for antibacterial activity
against Bacillus spp and Escherichia coli, as well as antifungal activity against
Aspergillus spp and Trichoderma spp. Based on the results that compound 2-
(CF3 and Cl groups at the 3rd and 4th positions respectively), 3,4-(OCH3 group
attached to the 4th position), 7- (CH3 group attached to the 2nd and 3rd position),
and showed inhibitory effects against Bacillus spp with the zone of inhibition ranging
from 6.66 – 9.83 mm. The compounds 1- (Cl group attached to the 2nd position),
2, and 8- (CF3 group attached to the 4th position) showed inhibitory effects against
Escherichia coli with ZOI ranging from 7.83 – 10.66 mm. The compounds 1,3, and
5-(Br group attached to the 4th position) showed inhibitory effects against
Aspergillus spp with ZOI ranging from 6.5 – 6.83 mm. The compounds 2, 6- (Cl
the group attached to the 4th position), and 8 showed an inhibitory effect against
Trichoderma spp with ZOI ranging from 7.5 – 8.0 mm. Synthesized 3-nitro
isoindoline1, 3-dione was tested for antioxidant activity by using the DPPH assay.
Compound 2 showed moderate antioxidant activity. These 3-nitroisoindoline-1,3-
dione analogues could be synthesized and used as antibacterial and antifungal and
antioxidant agents.
