Please use this identifier to cite or link to this item: http://ir.lib.seu.ac.lk/handle/123456789/6721
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dc.contributor.authorFathima Nashath, Mohamed Nazeer-
dc.contributor.authorMohamed Mubarak, Ahamadeen Nagoor-
dc.contributor.authorNishantha Tissa Kumara, Arachchi Devayalage-
dc.date.accessioned2023-07-05T04:30:12Z-
dc.date.available2023-07-05T04:30:12Z-
dc.date.issued2023-06-30-
dc.identifier.citationJournal of Bangladesh Agricultural University, 21(2): p. 124-131en_US
dc.identifier.issn1810-3030-
dc.identifier.issn2408-8684-
dc.identifier.urihttp://ir.lib.seu.ac.lk/handle/123456789/6721-
dc.description.abstractMaize genetic diversity is utmost to develop new varieties conferred with favorable agronomic traits. Landraces are priceless resources that can serve to strengthen crop breeding programs. Hence, this study aimed to characterize 19 Sri Lankan maize landraces and one elite commercial variety Bhadra using SSR markers linked with insect-resistant traits. Using established procedures, genomic DNA extraction was done from immature maize leaves and PCR was performed utilizing bnlg1017, bnlg339, umc1021, umc1187, bnlg1346, bnlg1588, bnlg1556, umc1178, nc134, umc1688 and umc1045 primers. GenAlex and DARwin software were used for the data analysis. According to the results, a sum of 77 alleles was amplified with the maximum number of observed (13) and effective (11.28) alleles for bnlg1588. The highest gene diversity and PIC values were recorded in bnlg1588 (0.911 and 0.904 respectively), while the lowest was in umc1045 (0.640 and 0.581 respectively). Out of the total SSR primers used, bnlg1588, bnlg339, bnlg1346, and bnlg1017 linked with FAW-resistant traits were found to be more informative based on their genetic diversity parameters. The accessions SEU7, SEU11, SEU16, and SEU20 showed more genetic diversity compared to Bhadra, whereas SEU17 showed the lowest diversity. The dendrogram divided the 20 maize accessions into two main clusters with the mean similarity of 0.752. Similarly, the principle coordinate analysis explained 40.63 % of the genetic variation and grouped the maize accessions into two. Considering the genetic diversity parameters, the accessions SEU6 and SEU7 from cluster I and SEU9, SEU11, SEU16, and SEU20 from cluster II could be utilized for strategic maize breeding programs in Sri Lanka.en_US
dc.language.isoen_USen_US
dc.publisherBangladesh Agricultural University (BAU)en_US
dc.subjectCrop breedingen_US
dc.subjectGenetic diversityen_US
dc.subjectInsect resistanceen_US
dc.subjectLandracesen_US
dc.subjectSSR markersen_US
dc.titleCharacterization of Sri Lankan maize (zea mays l.) accessions using ssr markers associated with insect-resistant traitsen_US
dc.typeArticleen_US
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