Experimental and molecular docking studies on DNA binding interaction of derivative of N-Arylhydroxamic Acid

Abstract

Binding affinity towards DNA for small molecules such as derivatives of Hydroxamic acids is very important in the development of new therapeuticreagents The binding interaction of N- (o-chloro) phenyl benzoh ydroxamicacid (N-(o-chloro)PBHA) with Ct-DNA was measured by four methods, (i) UV absorption, (ii) fluorescence emission, (iii) viscosity and (iv)molecular docking. It showed that N-(o-chloro) PBHA-DNA complex has less absorption intensity than compound only. Significant enhancement of fluorescence intensity was observed for the N-(o-chloro) PBHA-DNA complex. The values of binding constant, Kb, is 7.87x 10-3 and Stern Volmer constant Ksv is 3.0x10-3 ng-1 μl were obtained by UV absorption and fluorescence spectral methods, respectively. The binding interaction trend was further confirmed by viscosity on–Ct-DNA complex which shows the increase in viscosity. The molecular docking of N-(o-chloro) PBHA with the DNA showed the groove binding nature. This observation further confirmed and supplemented the experimental results. The study revealed that the hydroxamic acid derivative seems to have promising anti-cancer drug like nature.