Please use this identifier to cite or link to this item: http://ir.lib.seu.ac.lk/handle/123456789/5618
Title: Effects of carbendazim on In vitro micropropagation and flowering of pepper elder herbal plant
Authors: Ali Ahmed, Abdul Bakrudeen
Lydia, Teoh
Musthafa, Muneeb M.
Seshadri, Vidya Devanathadesikan
Vidhyavathi, Vijay Lobo
M. M.T. Marikar, Faiz
Keywords: Additives
Flower bud
Plant growth regulators
Sucrose
Surface sterilization
Issue Date: 30-Jun-2019
Publisher: Faculty of Agriculture, University of Ruhuna
Citation: Tropical Agricultural Research and Extension, 22(1-2), pp.17–29
Abstract: The present study reports on application of carbendazim for micropropagation and in vitro flowering of Peperomia pellucida. In vitro multiplication of shoot tips and axillary nodal explants were used in 25-100 mgL-1 carbendazim in 70% alcohol treatment for 5 min in MS (Murashige and Skoog) medium and MS hormone medium The explants were cultured on MS medium supplemented with auxins, cytokinins and additives (Casin hydrolyte and glutamine) used to proliferate the shoots. The maximum number of shoots (21.5/shoot tip) were developed on MS medium supplemented with 2.0 mgL-1 KIN (Kinetin) 0.5 mgL-1 NAA (α- Naphthalene acetic acid), 100 mgL-1 casein hydrolysate and 100 mgL-1 glutamine additives. The highest number of roots per shoot (17.6) and highest rooting frequency (94.2%) were obtained when the adventitious shoots were inoculated on MS medium with 1.5 mgL-1 IAA (Indole-3-acetic acid). The highest number of shoot development (75.4 %) was achieved on MS medium supplemented with 2.0 mgL-1 KIN 2.0 + 0.5 mgL-1 ABA (Abscisic acid) with 5% sucrose. It was found that MS medium devoid of 1.5 mgL-1 IAA promote the root formation and maturation of in vitro flower in P. pellucida. Acclimatized plantlets were transferred to botanical garden and the characters compared with mother plant of P. pellucida.
URI: http://doi.org/10.4038/tare.v22i1-2.5484
http://ir.lib.seu.ac.lk/handle/123456789/5618
ISSN: 1391-3646
Appears in Collections:Research Articles

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